Droplet based microfluidics
Published on 27 March 2024
The present work performed in the department of chemical and biological engineering at the University of Sheffield, describes how the researchers used pressure-driven droplet microfluidics in developing a system of enzyme-encapsulated double emulsion droplets to obtain a spatiotemporal pH control.
The research summary written by Rana Maheen is based on the peer-reviewed article “A microfluidic double emulsion platform for spatiotemporal control of pH and particle synthesis” by Rana Maheen, Ahmad Raheel, and Annette Taylor. Their work was recently published in Lab on a chip, 2023, 23, 4504-4513.
For the reaction, the double emulsions were mixed with an equal volume of an external solution (ES) containing acetic acid (1 or 2 mM), glucose (0.2 M), and urea (0.04–0.07 M) and 1 μL of the mixed solution was immediately injected into a reaction chamber. We used pyranine as a fluoroprobe to observe the increase in pH due to the formation of ammonia inside the microreactors. For determination of the apparent pH in the droplets, a calibration curve of pH vs. ratio of fluorescence intensities F458/F405 was used (Fig. S4†). Analysis of images was performed with a combination of ImageJ and MATLAB (version R2020a).
Enzyme labeling and concentration determination:
Microfluidic reservoir solutions:
The urease reaction has been well studied in batch reactors, and the rate depends on the initial concentration of urea, enzyme, and acid [5]. With relatively high enzyme concentrations, the pH increases rapidly to pH 7 and then more slowly to pH ∼ 9; the enzyme has a bell-shaped rate–pH curve with a maximum at pH 7 (Fig. 3A and S6†). In the double emulsion droplets, Rana and co-workers obtained a distinct pH pulse (not observed in bulk systems) characterized by a lag time or induction time Tind before a sudden increase to the maximum pH (pHmax ∼ 8) and then a slow decrease to the steady-state pH, pHss ∼ 7 (Fig. 3B).
Here, we used the emulsion properties to control the pH-time profile in the droplets. The pH change rate in the droplets depended on factors including oil shell thickness relative to core size. In general, an increase in the pressure ratio PMF/PIS increases S/C, with smaller core and thicker shells. The clock time increased with increasing shell/core (Fig. 5A, B), and the maximum rate of change of pH decreased (Fig. 5C) due to the longer time taken for urea to cross the oil layer. Unlike in bulk solutions, the reaction can approach a steady state in pH with values below or above 7. The pHss increased with increasing S/C as the ammonia transport out of the droplet was reduced in emulsions with thicker shells (Fig. 5D).
Get a quote
Name*
Email*
Message
Newsletter subscription
We will answer within 24 hours
By filling in your info you accept that we use your data.
Collaborations
Need customer support?
Serial Number of your product
Support Type AdviceHardware SupportSoftware Support
Subject*
I hereby agree that Elveflow uses my personal data Newsletter subscription
How can we help you?
Message I hereby agree that Elveflow uses my personal data Newsletter subscription