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Cell confinement Pack

Cell confinement Pack

Cell compression for mechanical cell stimulation experiments
Mechanobiological cell response

Allows advanced molecular and morphological analysis of confined cells

Precise mechanical stimulus

Trigger mechanical stimulus by adjusting confinement in real time

Standard cell culture plate

Compatible with 35 mm petri dishes and flat surfaces

Easy to use

Fast experiment set-up with plug-and-play equipment

We combined the Elveflow pressure control technology with the state-of-the art 4D Cell Confiner to create the ideal experiment conditions for biomechanical analyses of cells and tissues.

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The Mechanobiology pack brings together 4Dcell‘s cell confinement technology with the best in pressure control from Elveflow.

A standard setup contains a pressure controller and a cell confiner to better mimic in vivo cell culture conditions. The Cobalt pressure controller from Elveflow generates stable pressure while the cell confiner from 4D Cell provides micrometric control of the culture microenvironment. 

The combination of cell compression and spatial control provides insight into cell behavior in confined spaces and under mechanical stress, increasing the translational value of in vitro cell-based assays.  

Advantages for mechanobiology studies

  1. Control both pressure & physical space: Ideal for mechanical stress assays.
  2. Allows fast switches between pressure settings: For real-time observation of cell responses to mechanical stimulus.
  3. Stable & pulseless pressure: Total and stable control over cell compression.
  4. Non-destructive cell assay: Retrieve your cells for molecular analysis.
  5. Instantaneous start and stop pressure: Rapid trigger and release confinement.

Our dedicated pack can be adapted for more complex and advanced experiments. Contact us for more information!

Contents of the Cell confinement pack

  • 1 x Cobalt autonomous microfluidic pump
  • 1 x Security tank
  • 3 x 4Dcell Confiner (PDMS suction cup)
    • PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinment is applied
  • 12 x Confinment slides:
    • 10 mm diameter glass cover slips containing the PDMS micro-structures (pillars)
    • Confinement heights from 1 to 20 µm (you may select up to 3 heights for your kit)
  • 1 x Tubing and connectors kit

mechanobiology pack

 

BIOLOGY-RELATED FIELDS OF APPLICATION:

  • Cell confinement assays
  • Dynamic cell behavior
  • Cell migration and cancer metastases
  • Mechanotransduction
  • Cell migration plasticity
  • Cell response to mechanical stress
  • Live cell imaging
  • Cell division
  • Mechanical-induced cell repair
  • Genomic instability during cell division
  • Wound healing and inflammation studies
  • Cell entrapment

Reviews:

The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications.  Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.

CONTENT

  • 1 x Cobalt autonomous microfluidic pump
  • 1 x Security tank
  • 3 x 4Dcell Confiner (PDMS suction cup)
    • PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinment is applied
  • 12 x Confinment slides:
    • 10 mm diameter glass cover slips containing the PDMS micro-structures (pillars)
    • Confinement heights from 1 to 20 µm (you may select up to 3 heights for your kit)
  • 1 x Tubing and connectors kit
  • 3 x PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinment is applied

Cell & biology webinars

 

Cell & biology videos

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