Double emulsion pack

Start from scratch and quickly master all key parameters for double emulsion production.

Double emulsions (DE) can be water-in-oil-in-water (W/O/W) or oil-in-water-in-oil (O/W/O) droplets. The immiscible liquids composition prevents contact with the outer environment, giving DE great protective capabilities for cargo [1]. Whether you are an expert or a beginner, we provide all you need to understand DE production.

Pressure-driven flow-control provides:

• high monodispersity
• high encapsulation efficiency
• high precision
• low reagent consumption
• high reproducibility

This pack is composed of the premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment set, you will be able to:

• have ultra-precise pressure-driven flow control
• have access to all the necessary elements to efficiently produce double emulsions
• produce highly monodisperse DE droplets (CV~5%) with a high encapsulation efficiency (over 80%).

All the pack items are adjustable to your laboratory infrastructure and experimental requirements.

Build your pack in three quick and easy steps:

1. Talk to our experts in encapsulation
2. Tell them what you want to do
3. Our experts will design a pack tailored to your needs   

 

W/O/W double emulsion

Below we demonstrate the reproducible production of stable double emulsions using droplet-based microfluidics technology and Elveflow instruments. We tested different bacthes of double emulsions produced with pressure-driven flow control for monodispersity, encapsulation efficiency, and stability over time.

The produced double emulsions were highly monodisperse (CV~5%) and presented an encapsulation efficiency of over 80% (Fig 1).

Figure 1: Encapsulation efficiency. a) Double emulsions with the intermediate phase stained with DiI, lipid-specific fluorescent dye (red), b) and encapsulated calcein (green). c) Double emulsions encapsulating liposomes (POPC, 100 nm) stained with DiI.

 

The stability assays demonstrate that about 50% of the DE remains stable after 5 days at room temperature (Fig 2).

Figure 2: Stability at room temperature (RT). Percentage of remaining DEs and Size STDEV through time at RT. Double emulsions remain stable at RT for at least 72 hours, with over 50% of them still present after 5 days. Orange line, DEs radius in µm. 

The double emulsions produced by our system are highly monodisperse with a high encapsulation efficiency and remain stable after several days at physiological temperature.

References:

1. B. F. B. Silva, C. Rodríguez-Abreu, and N. Vilanova, ‘Recent advances in multiple emulsions and their application as templates’, Curr. Opin. Colloid Interface Sci., vol. 25, pp. 98–108, Oct. 2016.
2. S. Ding, C. A. Serra, T. F. Vandamme, W. Yu, and N. Anton, ‘Double emulsions prepared by two–step emulsification: History, state-of-the-art and perspective’, J. Control. Release, vol. 295, pp. 31–49, Feb. 2019.
3. J. Petit, I. Polenz, J. C. Baret, S. Herminghaus, and O. Bäumchen, ‘Vesicles-on-a-chip: A universal microfluidic platform for the assembly of liposomes and polymersomes’, Eur. Phys. J. E, vol. 39, no. 6, pp. 1–6, Jun. 2016.