Optoreader

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OPTOREADER – Optical detection for microfluidics

FAST ACQUISITION

Up to 100 kHz

HIGH SENSITIVITY

Best sensitivity in the market, < 1 nM FITC

OPTICAL FIBER BASED

Compact, 1-step alignment

Optical Reader for Microfluidics: the OptoReader brings about the strength of duplex optical fiber measurement, high optical sensitivity and real-time processing capacity within a compact design. It offers the same performance as a microscope while being more cost effective. The use of optical fiber for both illumination and light collection largely frees up the space around the experiment and reduces the optical alignment to only one step.

COMPACT PLATFORM FOR FLUORESCENCE QUANTIFICATION

Attributes

  • High sample rate: 100 000 samples/s
  • High sensitivity: refer to the technical specifications
  • Lock-in detection capability: to eliminate constant background
  • Compact design: the use of optical fiber allows low footprint and versatile integration
  • Plug & Play: USB computer connection, empowered by the Elveflow software suite (ESI)
  • Interoperability: TTL source trigger

Application fields

Microfluidics, fiber optic sensor, analytical or bio chemistry, biology, real-time inspection (food, pharmaceutics, etc), aerospace, etc

Optical detection microfluidic

Multiple configuration possibilities:

  • Single detection: Measurement of fluorescence or reflection
  • Dual detection: Simultaneous measurement on two detection channels
  • Multiple color excitation: beta, up to 3 colors

with

  • Custum filter set: 9 types of fluorophores
  • Custom excitation wavelength: from UV to IR

And many other options:

  • High fluorescence sensitivity: cooled detector, noise lower by a factor 10.
  • LED or LASER source
  • Alignment platform: Micrometric alignment table allowing beam alignment in a microfluidic chip.
  • Inspection camera
  • Customer inspired: Contact us for original propositions inspired by your needs!

OptoReader options

PROPERTIES

SPECIFICATION

Unity

EXCITATION

Excitation wavelength

365, 470, 530, 590, 625

nm

Fluorescence filter Set

DAPI, FITC, TRITC, Texas Red, Cy3, Cy5, etc

 

Light output
intensity

0-1.5

mW

Pulse duration (pulse mode)

10-107

µs

Modulation (Lock-in mode)

0.05 -10

kHz

ACQUISITION

Acquisition frequency

0-100

kHz

Acquisition resolution

16

bits

Typ. acquisition dynamic

84 dB

dB

 

Reflection

Fluorescence

 

Measurement range

0 – 12

0 – 0.12

µW

Noise equivalent power

10-5

Base

3 x 10-15

W/Hz1/2

 

Hypersensitive (Analog)

0.3 x 10-15

 

 

Hypersensitive (Digital)

10

kcps

Photoelectric sensitivity (532 nm)

0.5 x 106

Base

2 x 109

V/W

 

Hypersensitivity

1010

V/W

Equivalent fluorescence background

(Specified on normal mode, virtually zero in lock-in mode)

 

< 1 nM FITC

 

OPTICS

Fiber core diameter

50, 100, 200, 400

µm

Numerical aperture

0.22 – 0.6

 

Focalized spot diameter

35, 75, 150, 300

µm

MECHANICS

Dimension

35 x 25 x 12

cm3

Detection limit : <1nM FITC (available option with photon counting capability).

Other wavelength and focalized spot diameter available on request.

These specifications are not contractual and can be changed without notice.

 

USB flow sensor software module

Microfluidc flow control software HeartBeat-ESI-2015Thanks to an intuitive interface, the Elveflow® Smart Interface enables the use of Elveflow® instruments from the simplest commands for beginners to the most complex manipulations for experts… read more

 

Control your experiments through C, Python,  Matlab®, Labview® or the Elveflow® Smart Interface. The Elveflow® Smart Interface is a software application offering all the functionalities that microfluidicists need.

T. Houssin, J. Cramer, R. Grojsman, L. Bellahsene, G. Colas, H. Moulet, W. Minella, C. Pannetier, M. Leberre, A. Plecis and Yong Chen. Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections. Lab on a Chip, Feb 2016, DOI: 10.1039/c5lc01459j

Application note Detection of fluorescent droplets using the Optoreader

droplet generation fluorescence measurement optoreader Ob1

This diagram shows the microfluidic setup used for Detection of fluorescent droplets using Optoreader.

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