contact@elveflow.com+33(0).184.163.807+1(414)-406-4343 Elveflow is an Elvesys brand, an international microfluidics innovation center with high level researchers dedicated to microfluidics and organ-on-a-chip.
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Microfluidics for the diagnosis and treatment of tuberculosis
Explore how microfluidics is revolutionizing cosmetic research from formulation to toxicology with ethical, precise, and scalable testing.
The Dynamics of Fungal Spore Dispersal: Insights from Microfluidic Models
Air bubbles are among the most recurring issues in microfluidics. Because of the micrometric dimensions of the tubes and channels
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03_injection_pack_new_mux_distrib_valve_12_elveflow_microfluidics [status] => inherit [uploaded_to] => 13079 [date] => 2021-03-09 16:19:35 [modified] => 2023-12-15 16:37:32 [menu_order] => 0 [mime_type] => image/png [type] => image [subtype] => png [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2020/03/03_INJECTION_PACK_New_Mux_Distrib_valve_12_ELVEFLOW_MICROFLUIDICS.png [thumbnail-width] => 150 [thumbnail-height] => 93 [medium] => https://www.elveflow.com/wp-content/uploads/2020/03/03_INJECTION_PACK_New_Mux_Distrib_valve_12_ELVEFLOW_MICROFLUIDICS.png [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2020/03/03_INJECTION_PACK_New_Mux_Distrib_valve_12_ELVEFLOW_MICROFLUIDICS.png [medium_large-width] => 768 [medium_large-height] => 475 [large] => 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The pack is compatible with different instruments, and the entire setup is controlled via a single software. Advantages This pack includes our premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment, you will be able to:
An all-inclusive Pack to generate droplets out-of-the box. We setup a dedicated set of instruments, controlled by a unique software, to ease the droplet generation process.
It brings the many benefits of microfluidics, such as excellent monodispersity, reproducibility and scalability to your daily work in order to achieve great science.
It has never been easier to start generating droplets following a step-by-step User Guide and quickly master the process with the same system.
Schematics outlining multiplexed RNA and protein detection methods by Mayr U. et al.
This all-included, user-friendly, customizable, and automatable instruments pack is ideal for MERFISH/seqFISH/seqFISH+ experiments but can be easily adapted to other applications.
The pack is compatible with different instruments, and the entire setup is controlled via a single software.
This pack includes our premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment, you will be able to:
*The sequence scheduler automatizes the platform to flow a large number of different solutions easily. Our experts can help you integrate your experiment using TTL triggers or direct software integration via SDK.
This pack is designed to be:
It allows you to perform multiplexed fluorescence in situ hybridization at a microfluidic scale, significantly decreasing the cost of each experiment by reducing the volume of the reagents required.
Elveflow microfluidic platforms are perfectly suited for long-term experiments with excellent stability and no risk of potentially harmful pressure spikes.
All the pack items are adjustable to your laboratory infrastructure and experimental requirements.
Fig 1: This is an illustrative setup example. The solutions’ number and volume can be adjusted according to the experimental requirements and protocol.
seqFISH is a highly sensitive technique that accurately detects low-copy number genes often not detected with single-cell RNAseq or immunostaining. Additionally, reverse transcription or PCR amplification often biases quantification in RT-PCR and RNA sequencing. Because seqFISH can be applied to any tissue type without preselecting genes, it enables the unbiased discovery of novel genes relevant to certain biological phenomena.
seqFISH generates accurate in situ quantification of mRNA levels [1]
SeqFISH and MERFISH use probe detection for single-cell spatial transcriptomics [1][2][3].
At first, in situ hybridization is done with one set of fluorescent FISH probes and a labeling dye. DNase is then used to remove the fluorophores, and the mRNA is hybridized with the identical FISH probes again but with a different labeling dye. The several rounds of hybridization and other dyes allow the barcoding of several genes in a single cell [4].
SeqFISH+ is the improved seqFISH technique ideal for spatial and biological processes studies of cells. It combines seqFISH with a confocal microscope generating super-resolution imaging and multiplexing of 10,000 genes in a single cell [5].
Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) is the improved single-molecule Fluorescence In Situ Hybridization (smFISH). The method massively parallelizes and simultaneously spatially identifies hundreds of thousands of RNA species. In addition, this method detects errors that can then be corrected in an error-robust way thanks to the use of some unassigned binary barcode. This is the main difference compared to seqFISH, which is coded in a color sequence [6].
Lab-on-chip technologies and microfluidic platforms improve seqFISH and MERFISH methods by reducing the cost and the experiment time, providing automation to the process, and improving reproducibility [7].
*Chips are available in several materials, heights, widths, lengths, and shapes, with different numbers of inlets/outlets, and are compatible with different FISH methods. We can advise you on different chips to fit this pack.
Build your pack in three quick and easy steps:
Using microfluidics is the most efficient method to perform MERFISH (Multiplexed Error-Robust Fluorescence In Situ Hybridization) or seqFISH (sequential Fluorescence In Situ Hybridization) and observe multiple genes and their spatial configuration because:
This pack can be combined with other microfluidic steps before this fluorescent in situ hybridization setup. For example, for single-cell isolation, you can use microfluidic single cell encapsulation [1].
Microfluidics can also be used for the method called MA-FISH, which uses oscillatory flows of diluted probe solutions or to perform barcoding (DBiT-seq).
Elveflow has been the microfluidic flow control expert company for more than 10 years and can provide its state-of-the-art expertise in biology and engineering, thus, being the perfect partner for you to transition to microfluidics.
Schematics outlining multiplexed RNA and protein detection methods. [1]
[1] Mayr U., Serra D., Liberali P. Exploring single cells in space and time during tissue development, homeostasis and regeneration. Development, 2019, 146(12),
The Lipid Nanoparticle Synthesis Pack includes all the parts needed to easily synthesize your lipid nanoparticles with high monodispersity, production rate and reproducibility for the optimal encapsulation of your mRNA or siRNA molecules.
Microfluidics presents tremendous advantages to overcome the issues encountered with common Liposome bulk preparation. Being characterised by laminar flow conditions and diffusive mass transfer, microfluidics allows for the production of liposomes with excellent control over size and lamellarity. In addition, it enables in situ monitoring of lipid nanoparticles formation process, continuous production and straighforward scale up.
Based on our high accuracy OB1 flow controller, this solution reduces lipid nanoparticle synthesis time and allow to work at small and large volumes (µL to L) while optimizing reagent consumption.
For several years, we have worked hand-in-hand with Inside Therapeutics (InsideTx) to deliver advanced microfluidic solutions for nanoparticles and RNA-LNP formulation. Together, we’ve combined our cutting-edge microfluidic flow control capabilities and Inside Therapeutics’ deep formulation expertise to bring you TAMARA, the most advanced microfluidic-based nanoparticle manufacturing system on the market.
TAMARA is based on a bottom-up microfluidic formulation approach to precisely control nanoparticle formulation conditions. The goal was simple: combine the best of microfluidics with user-friendly operation so researchers can focus entirely on drug development, not microfluidic technicalities.
That’s exactly what TAMARA delivers: by embedding Elveflow’s high-performance pressure-driven flow control technology within an intuitive platform, TAMARA makes optimal formulation conditions easily accessible, without needing to be a microfluidic expert.
With its near instant response time and zero sample loss, TAMARA eliminates common issues that limit regular microfluidic systems such as head and tail losses. Its broad volume range supports the full drug development pipeline from screening to preclinical studies.
Its integrated microfluidic device is both reusable and affordable, making it an ideal tool for screening development and meeting quality requirements.
Traditionally, nanoparticle formulation relied on top-down techniques such as extrusion, high-pressure homogenization, or sonication. While effective at scale, these techniques often suffer from broad particle size distribution and poor batch-to-batch reproducibility.
In contrast, bottom-up approaches, especially nanoprecipitation via microfluidics, offer enhanced control over formulation conditions. Microfluidics is particularly promising for RNA-loaded lipid nanoparticles (RNA-LNPs) due to:
Overview of the nanoparticle and RNA-LNP formulation methods
More info: RNA-LNP manufacturing methods by InsideTx.
Typical RNA-LNP composition used in the mRNA-LNP covid vaccine (Moderna formulation)
RNA-LNPs are formed by rapid mixing of two phases:
When mixed, lipids self-assemble into nanoparticles due to solubility changes. Microfluidics enables precise control of this process, improving reproducibility and reducing waste.
Note: Downstream steps such as solvent removal or sterilization are typically performed post-synthesis and are not part of TAMARA’s integrated process.
Thanks to their flexibility, RNA-LNP can be used for a wide range of vaccines and therapeutics applications
RNA-LNP technology is widely used to develop vaccines that generate strong immune responses against infectious diseases. By delivering mRNA encoding a disease-specific antigen , (viral or bacterial protein) the immune system is trained to recognize and combat the actual pathogen.
This approach showed its potential with the COVID-19 mRNA vaccine but can also be used for other infectious diseases such as Malaria, HIV… While mRNA is the most common payload, other RNA types like self-amplifying RNA (saRNA) and circular RNA (circRNA) are also being explored to enhance efficacy and stability.
By delivering tumor-specific RNA sequences that encode neoantigens, RNA-LNPs can prime the immune system to recognize and eliminate cancer cells . This personalized approach is tailored to the unique mutation profile of each patient’s tumor, opening the door to highly targeted immunotherapies.
RNA-LNPs are increasingly used to deliver therapeutic RNA into cells to treat genetic disorders. This includes two key therapeutic strategies:
mRNA-Based and siRNA Therapies
These approaches are designed for transient protein expression or temporary gene silencing , making them ideal for conditions where short-term therapeutic effects are sufficient. A prominent example is in vivo CAR-T therapy in oncology, where mRNA-LNPs deliver genetic instructions for chimeric antigen receptor (CAR) expression directly in the patient’s T cells. In this context, gene delivery remains a critical challenge, primarily due to the liver tropism of lipid nanoparticles. Overcoming this barrier often involves surface functionalization of lipid nanoparticles to improve targeting specificity.
Another example is Onpattro® (patisiran) by Alnylam, a siRNA-LNP based therapy that targets and silences the mRNA responsible for producing abnormal transthyretin (TTR) protein in hereditary transthyretin-mediated amyloidosis (hATTR).
LNPs are also used to deliver CRIPSR-CAS9 components for permanent gene correction, offering a safer, non-viral alternative to traditional RNA delivery methods such as electroporation or lentivirus. This approach holds promises for treating monogenic diseases at their root cause. Additionally, RNA-LNPs can be adapted to deliver antisense oligonucleotides and other gene-silencing tools to precisely regulate gene expression.
Check out our recent review to know more about CRISPR-CAS9 and its relationship with microfluidics !
This list is not exhaustive. As this field is very active, there are many other applications.
RNA-LNP therapeutics and vaccines have revolutionized modern medicine, yet several key challenges remain before their full potential can be realized. Beyond achieving therapeutic efficacy, enhancing stability, immunogenicity, and extra hepatic delivery are central to advancing next-generation RNA therapeutics. Current efforts focus on three major areas:
1.Targeted Delivery and Biodistribution Control One of the most pressing challenges is improving biodistribution , where and how the LNPs travel and accumulate in the body. Current LNPs show a strong liver tropism , which is not always ideal for vaccines or non-hepatic diseases. Improved biodistribution enhances therapeutic efficacy and safety, especially in systemic applications and repeated dosing scenarios. Improved targeting can be achieved using two different approaches, passive and active targeting:
For more information on this topic, see the detailed review of RNA-LNP active and passive targeting techniques from InsideTx.
2.Mitigating PEG Immunogenicity and Improving Repeat Dosing PEGylation is commonly used to prolong LNP circulation time, but it also poses risks. Anti-PEG antibodies can develop, reducing efficacy upon re-administration and increasing the risk of hypersensitivity reactions. Alternatives such as PEG substitutes , biodegradable polymers , or zwitterionic coatings are under investigation to preserve stealth properties while minimizing immune responses.
3.RNA-LNP vaccine stability The cold chain requirement for mRNA vaccines is a major barrier to global distribution. In a first step, adjusting the lipid mix can significantly enhance the thermal and chemical stability of RNA-LNPs. This involves screening large libraries of lipid combinations, an area where microfluidics excels , thanks to its ability to test hundreds of compositions using minimal sample volumes. Other approaches such as lyophilization , or freeze-drying, can stabilize RNA-LNPs for storage at room or refrigerator temperatures. However, maintaining nanoparticle structure and RNA integrity during rehydration is complex.
From oncology to immunity and rare disease treatment, RNA-LNPs are transforming what’s possible in drug development. Whatever your application, the Elveflow and InsideTx teams are here to help, feel free to reach out to us to discuss your project.
Nanoparticle size is one of the most critical characteristics, influencing biodistribution , cellular uptake , and overall therapeutic performance . The size of the LNPs is primarily governed by the mixing speed between the solvent and aqueous phase triggering the LNP formation. Faster microfluidic mixing leads to smaller particles, while slower mixing results in larger ones, as illustrated in the below graph.
Influence of mixing speed and conditions on RNA-LNP size
To achieve precise and tunable size control, microfluidic devices typically incorporate efficient microfluidic mixing structures, such as baffle and herringbone micromixer , two of the most popular microfluidic chaotic mixers. These micromixers allow users to control nanoparticle size by simply tuning the TFR ( Total Flow Rate , or sum of the aqueous and solvent flow rates ), making size optimization intuitive and repeatable.
Furthermore, FRR (Flow Rate Ratio, or the ratio of the aqueous flow rate over the solvent flow rate) can also be tuned to further control the nanoparticle characteristics.
Illustration and image under a microscope of a baffle and a herringbone microfluidic mixers used for RNA-LNP formulation.
In practice, the graph below illustrates the impact of the formulation conditions on nanoparticle characteristics. It can be noticed that both the lipid composition and the formulation parameters (here the TFR) greatly impact the nanoparticle size, reinforcing the importance of the high level of control offered by microfluidics.
Influence of TFR and composition on LNP size and PDI in a Herringbone microfluidic mixer
While size is critical, other formulation parameters, such as lipid ratios , ionizable lipid choice, and process conditions, have a direct impact on other Critical Quality Attributes (CQAs) like encapsulation efficiency, morphology , and in vivo performance . This complexity underscores the importance of screening a wide range of formulation conditions, particularly during early phase development. This is precisely where microfluidics excels , enabling the efficient production and testing of numerous RNA-LNP formulations at ultra-low volumes, accelerating development while minimizing the use of expensive RNA material.
One of the greatest strengths of microfluidic systems is their adaptability. The same microfluidic platform can be used to produce a wide array of nanoparticle types, from liposomes to complex RNA-LNP formulations, across a broad range of working volumes. This flexibility makes microfluidics ideal not only for screening and optimization but also for producing material for preclinical animal studies.
In practice, microfluidic formulation systems can be deployed in two main ways: homemade system, such as the LNP pack, a custom system including the OB1 pressure controller and flow sensors from Elveflow, and more integrated platform, such as TAMARA from Inside Terapeutics:
Liposome and Lipid Nanoparticle Synthesis Pack related videos
Liposome and Lipid nanoparticle synthesis The Liposome and Lipid nanoparticle synthesis pack is designed to suit your application requirements. It contains at least two pumping channels to push the two chemical solutions needed to perform the Liposome and lipid nanoparticle synthesis process inside at least one herringbone micromixer chip. Lipid nanoparticle (LNP), solid lipid nanoparticles (SLN) and nanoliposomes can be synthesized using this instrument pack. Microfluidics chips are used in this system to induce the mixing of your two solutions at a microfluidic scale. The first liquid contains the lipids in ethanol and the second one is the aqueous solution with possibly the hydrophilic load that will be encapsulated inside the newly formed LNP such as siRNA or mRNA for example (see the application tab). Two different chip designs can be provided with this pack, depending on your requirements: Flow focusing chips for smooth control of your low volumes/flow rates Staggered herringbone which induces a chaotic mixing for larger volumes/flow rates. The production can be easily scaled up by increasing the volumes and flow rates, and/or parallelizing several micromixers instead of one, thus increasing the overall throughput of the system while maintaining monodispersity and yield. The stability and the speed of the reaction directly depend on the flow rates of each fluid and their ratios in the microfluidic channel. The flow is created by the Elveflow OB1 mk3+ flow controller and the flow rates are measured and regulated thanks to flow rate sensors ( MFS or BFS series) allowing a very high accuracy and stable flow control. The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guarantees the best possible LNP monodispersity and reproducibility. Furthermore, the lipid nanoparticle synthesis process can be automated thanks to the software controlling the Elveflow instruments. Pressure-driven flow control systems are well-suited for Liposome and Lipid nanoparticle synthesis compared to peristaltic or syringe pumps as they offer the most pulsless flow and can be easily adapted for small and large volumes.
The Liposome and Lipid nanoparticle synthesis pack is designed to suit your application requirements.
It contains at least two pumping channels to push the two chemical solutions needed to perform the Liposome and lipid nanoparticle synthesis process inside at least one herringbone micromixer chip. Lipid nanoparticle (LNP), solid lipid nanoparticles (SLN) and nanoliposomes can be synthesized using this instrument pack.
Microfluidics chips are used in this system to induce the mixing of your two solutions at a microfluidic scale. The first liquid contains the lipids in ethanol and the second one is the aqueous solution with possibly the hydrophilic load that will be encapsulated inside the newly formed LNP such as siRNA or mRNA for example (see the application tab).
Two different chip designs can be provided with this pack, depending on your requirements:
The production can be easily scaled up by increasing the volumes and flow rates, and/or parallelizing several micromixers instead of one, thus increasing the overall throughput of the system while maintaining monodispersity and yield.
The stability and the speed of the reaction directly depend on the flow rates of each fluid and their ratios in the microfluidic channel. The flow is created by the Elveflow OB1 mk3+ flow controller and the flow rates are measured and regulated thanks to flow rate sensors ( MFS or BFS series) allowing a very high accuracy and stable flow control. The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guarantees the best possible LNP monodispersity and reproducibility. Furthermore, the lipid nanoparticle synthesis process can be automated thanks to the software controlling the Elveflow instruments.
Pressure-driven flow control systems are well-suited for Liposome and Lipid nanoparticle synthesis compared to peristaltic or syringe pumps as they offer the most pulsless flow and can be easily adapted for small and large volumes.
Configure your microfluidics lipid nanoparticle production The fluidic 187 herringbone chip from microfluidic ChipShop is composed of three separate channels that are 200 µm deep and 600 µm wide. The two inlets for a single channel are 300 µm wide and the single outlet is 600 µm wide. The microfluidic chip is available in polycarbonate (PC) or Zeonor cyclo-olefin copolymer (COP) materials: these materials are optically transparent and harder than the classically used PDMS. Herringbone micromixer, ChipShop Fluidic187 Alternatively, you can choose a flow focusing micromixer wich will allow for nanoparticle nucleation thanks to diffusion at the interface of the phases. The microfluidic platform flexibility can be increased by adding a MUX Distribution 12:1 valve after the reservoirs that allows switching between up to 12 different solutions in an instant. This can be, for example, used to quickly change the load of the lipid nanoparticle! A broad range of reservoirs are compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100 mL bottles. It is also possible to add pumping channels on the OB1 pressure control pump to increase the number of parallel micromixer channels further. Contact our experts to answer any questions about this lipid nanoparticle synthesis pack and how it can match your specifications!
The fluidic 187 herringbone chip from microfluidic ChipShop is composed of three separate channels that are 200 µm deep and 600 µm wide. The two inlets for a single channel are 300 µm wide and the single outlet is 600 µm wide. The microfluidic chip is available in polycarbonate (PC) or Zeonor cyclo-olefin copolymer (COP) materials: these materials are optically transparent and harder than the classically used PDMS.
Herringbone micromixer, ChipShop Fluidic187
Alternatively, you can choose a flow focusing micromixer wich will allow for nanoparticle nucleation thanks to diffusion at the interface of the phases.
The microfluidic platform flexibility can be increased by adding a MUX Distribution 12:1 valve after the reservoirs that allows switching between up to 12 different solutions in an instant. This can be, for example, used to quickly change the load of the lipid nanoparticle!
A broad range of reservoirs are compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100 mL bottles. It is also possible to add pumping channels on the OB1 pressure control pump to increase the number of parallel micromixer channels further.
Contact our experts to answer any questions about this lipid nanoparticle synthesis pack and how it can match your specifications!
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MK4 ) [avantages] => Array ( [liste] => Array ( [0] => Array ( [icon] => icon-arrow-right [titre] => PRESSURE AND VACUUM CONTROL [texte] => Accurately control pressure to push and pull liquid ) [1] => Array ( [icon] => icon-arrow-right [titre] => FLOW CONTROL [texte] => Pair it with a flow sensor for accurate flow control ) [2] => Array ( [icon] => icon-arrow-right [titre] => UNMATCHED PERFORMANCE [texte] => High precision and responsiveness for your flow regulation ) [3] => Array ( [icon] => icon-check [titre] => MICROFLUIDIC AUTOMATION [texte] => Automate and control your microfluidic experiment ) ) ) [introduction_texte] => The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications. Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers. 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Cutting-edge Microfluidic Flow Controller Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs. Check out our OB1 animation here! The best performance on the market: Piezoelectric Technology The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market. Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want. Get the fastest flow rate control when paired with a flow sensor Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate. Full control software, SDK, and UART communication Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC. OEM version available The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution. [image] => ) [applications] => Array ( [texte] => Applications of Pressure-Driven Flow Control Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains. Droplet & Bead Generation Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack. Biology & Cell Culture Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study Flow Control & Mixin Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup Analytical Chemistry & Imaging Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC Industrial Applications Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review Cosmetic Formulation & Testing Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications Integrated Systems Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review Videos Publications For all publications featuring our products, please click here. For all application notes based on our product, please click here. For reviews of our product, please click here. ) [specifications] => Array ( [texte] => This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller. OB1 MK4 CHANNEL PRESSURE RANGE 0 to 200 mbar1 (0 to 2.9 psi) 0 to 2,000 mbar1 (0 to 29 psi) 0 to 8,000 mbar1 (0 to 116 psi) -900 to 1,000 mbar1 (-13 to 14.5 psi) -900 to 6,000 mbar1 (-13 to 87 psi) Pressure stability (2) 0.015% FS 30 µbar (0.0004 psi) 0.005% FS 100 µbar (0.0014 psi) 0.006% FS 500 µbar (0.007 psi) -900 to 500 mbar: 0.005% FS 100 µbar (0.0014 psi) 500 to 1,000 mbar: 0.007% FS 150 µbar (0.0021 psi) -900 to 2,000 mbar: 0.005% FS 350 µbar (0.005 psi) 2,000 to 6,000 mbar: 0.007% FS 525 µbar (0.008 psi) Response time (3) down to 10 ms Settling time (4) down to 50 ms Minimum pressure increment 0.006% FS 12 µbar – 0.00017 psi 0.006% FS 120 µbar – 0.0017 psi 0.006% FS 480 µbar – 0.007 psi 0.0064% FS 120 µbar – 0.0017 psi 0.0061% FS 420 µbar – 0.006 psi Pressure supply 1.5 bar to 10 bar Non corrosive, non explosive, dry and oil-free gases, e.g., air, argon, N2, CO2, … Input vacuum (5) / / / Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Liquid compatibility Non contact pump Any aqueous, oil, or biological sample solution. Non-contractual information, may be changed without notice (1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only Flow control Flow sensor compatibility Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available Flow rates From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series) Liquid compatibility Non-contact pump Any aqueous, oil, or biological sample solution. Control & monitoring Software control Elveflow Smart Interface – Windows 7, 8, 10, both 32 and 64 bit versions supported Software Development Kit Libraries available: Matlab, Python, LabView, C++ – Windows 7 & 10, both 32 & 64 bit versions supported Serial/UART communication protocol on request Data management Possibility to log and extract data (CSV): channel and sensor detailed information using ESI Input profiles Possibility to load profiles: ramp, sine, triangle, square, or custom Automation Generate step-by-step sequences using the ESI built-in sequence management Log and export custom configurations (CSV) Screen LCD screen showing pressure and sensor flow rate in real time Electrical Specifications Input Voltage (V) 24V Typical Power (W) 12W Provided Power Supply Specifications Supply Voltage Range (V): 100 to 240 VAC Supply AC Frequency (Hz): 50 to 60 Hz Maximum Output Current (A): 1.5 A Maximum Output Power (W): 36W Interface USB Type B Communication Type Serial Software Control ESI Sensor Connection One M8 4-pins connector per channel Compatibility Elveflow sensors: MFS, MPS, MFP, MBD Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage Triggers Input and Output TTL signal 0V or 5V Other Casing dimensions (length x width x height): 240 x 223 x 80 mm Weight 1.4 kg to 2.9 kg Non-contractual information, may be changed without notice [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => Array ( [0] => Array ( [question] => What pressure range should I choose? [reponse] => The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range. In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures. ) [1] => Array ( [question] => Can I achieve direct flow control? [reponse] => By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value. The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor). ) [2] => Array ( [question] => Can I connect a Pressure Sensor to the OB1? - [reponse] => Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave). ) [3] => Array ( [question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ? [reponse] => Yes, you will need a pressure source to work with the OB1 pressure controller. You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1. If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1. ) [4] => Array ( [question] => How to install the OB1? [reponse] => Click here Unboxing video ) ) ) [more] => Array ( [more_title] => Software [texte] => Software ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code. The OB1 control window allows to:
The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications.
Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers.
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.
Check out our OB1 animation here!
The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want.
Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.
Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.
The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution.
Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.
Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup
Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.
Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup
Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems
Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example
Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example
Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study
Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack
Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup
Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application
Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research
Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC
Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review
Cosmetic Formulation & Testing
Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications
Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review
For all publications featuring our products, please click here.
For all application notes based on our product, please click here.
For reviews of our product, please click here.
This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller.
Non-contractual information, may be changed without notice
(1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
Click here
Unboxing video
ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.
The OB1 control window allows to:
For more details about Pressure driven flow control, please read this application note.
By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision.
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
Add and control 2-way and 3-way valves anywhere in your microfluidic setup
With Elveflow’s MUX Wire, you can plug up to 8 microfluidic valves – either Elveflow’s 2-way or 3-way valves or your own. Each valve is connected individually to the MUX Wire through mini-USB cables that provide power and trigger signal.
The compact design and ease-of-use of the MUX Wire and the valves make them suitable for any microfluidic setups and OEM integration.
The MUX Wire version 3 (V3) microfluidic valve controller works perfectly with all of Elveflow’s microfluidic valves and devices through the same software interface.
It is equipped with internal and external trigger connectors that synchronize it with the other equipment in the setup, such as microscopes or analytical instruments.
The LED indicator on the microfluidic valve controller displays each valve’s type and status, facilitating the use, visualization, and control of all the valves in your setup.
Smart valves can be independently or simultaneously controlled through Elveflow’s software interface (ESI). The ESI automatically detects these valves once plugged into the Mux Wire.
Three microfluidic valve models are available. The valve model relates to the supported pressure – high pressure (until 6 bars), low pressure (until 3 bars), and low volume (for low internal volume requirements).
The valve type indicates the flow path and the normally open (NO) or closed (NC) state of the valve. The V3 range offers three types of valves: 2/2 NO, 2/2 NC, and 3/2 valves.
The combination of valve models and valve types gives different possibilities to fulfill specific setup requirements.
Contact our experts for the valve that perfectly fits your needs!
Elveflow’s smart valves have been carefully selected for their convenience and performance to suit most microfluidic applications. Our valves provide 20ms or less switching time, low internal volume, high chemical compatibility, and no disruption flow.
It is possible to power the MUX wire, plug valves, and control them manually via the push button on the valve. This way, the system works in a standalone mode, without the ESI or a computer!
The 3/2 way valve is ideal for bidirectional control: it can be used to choose between two inlets and one outlet or one inlet and two outlets.
For all the publications using our products, please click here.
For all the application notes and reviews based on our product, please click here.
Non-contractual information. May be changed without notice.
1 Using ESI: 175 ms / using SDK: 5 ms 2 Volume inside the system from entrance to exit 3 Volume trapped in the system (dead end), not fully removed and relies on diffusion to clear out. 4 Always make sure your fluid is compatible with the wetted materials before starting any experiment.
ESI is Elveflow’s advanced software interface that is built to make every experimenter’s life better. It is perfectly adapted from simple setups to very advanced setups and workflow automation. It integrates several modules that make time-consuming and painful tasks very simple. Elveflow also provides Software Development Kit (SDK) libraries to integrate Elveflow systems using your own code.
Some additional features are available when connecting the MUX to the ESI. The new ESI MUX interface offers detailed information about the type and state of the valves connected to the instrument.
Each valve displays:
The smart valves are automatically added once connected to the MUX Wire. A custom valve or an older valve will not be automatically recognized, you will have to add it manually in the software.
The MUX wire V3 allows you to activate your valves in two ways:
Automate your experiment and switch from one configuration to another through the ESI Sequencer:
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