Beta Pack

dielectrophoretic sorting pack

what is it?

We have sought to create a system that provides the necessary conditions to perform experiments based on the dielectrophoretic sorting phenomenon. The Dielectrophoretic sorting pack is a system that use an electric field to manipulate and sort cells based on their charge and mobility. The main objective of this pack is to create a scalable system that allows the use of microfluidic chips to perform sorting of different particles or cells.

A microfluidic chip based on this principle works because samples containing cells are introduced into a microfluidic chamber. Through this chamber an electric field will be applied where the cells will be repelled or attracted. For this reason, by controlling the parameters of the electric field, cells can be sorted with adequate accuracy.

An all-in-one pack to easily separate your cells The use of microfluidics for dielectrophoresis separation (DEP) is a promising technique due to its great contributions to biotechnology, medical diagnostics and other areas related to the manipulation of particles and cells at the microscale. It also offers many advantages, among which are the following: key advantages: Increased precision in the accurate control of the flow rate thus ensuring better particle and cell handling throughout the process. High separation efficiency and selectivity ensures high purity. Low cost due to savings in sample and reagent costs High automation by reducing manual intervention and the possibility of human error. High capacity for integration with other analytical or detection techniques.

Why use microfluidics for the separation of cells?

The use of this beta pack will allow your microfluidic system to perform a controlled dielectrophoresis (DEP) separation process, which will give you the opportunity to make important advances in biomedical research. For example, it allows the separation of cells and particles at the microscale, which is necessary for the study of complex biological processes. It also has an excellent application in medical diagnostics as it favors the sensitive and selective detection of biomarkers in patient samples, which can improve the accuracy of diagnosis and treatment. Likewise, in personalized medicine, this technology can improve treatment efficacy and reduce unwanted side effects. In the case of cell therapy, it will enable the separation and manipulation of cells for tissue engineering, gene therapy and other regenerative medicine applications.

Overall, this beta pack will allow you to run your microfluidic system to achieve cell separation with high efficiency, cost reduction and large scale production.

Overview of the biosensor setup

How to choose the better configuration of the pack?

If you already want to start testing your microfluidic system you can try the configuration that this pack has for you.

SETUP AND CONTENT:

First, in order to perform a dielectrophoresis separation it is necessary to have the following components: Microfluidic device: The channels and cavities of the microfluidic chip should be designed for DEP separation. Power supply: The separation requires to apply an electric field across the microfluidic device with a specific voltage and frequency to produce the desired DEP effect. Electrode: A set of electrodes is required to apply the electric field in the microfluidic device. Sample: The sample can be a suspension of particles or cells in a liquid that is introduced into the microfluidic device. Detection system: A detection system may be required to monitor the separation (e.g. Raman spectroscopy).

MODEL of the setup of the pack:

One of the examples of packs for cell or particle separation that allow the injection of several samples with different characteristics and the use of a buffer substance will be presented below.

For example, in the first setup, it is possible to observe that there will be two inlet and two outlet on the microfluidic chip. The function of the first inlet is to introduce the buffer substance to the chip. Subsequently, this substance will be mixed with the second inlet substance. This second substance will be the sample containing the different cells or particles to be separated. When these two substances are mixed, they will pass through an electric field that will allow them to separate according to the variables you want.

Therefore, the waste from the experiment will come out of one outlet and the type of cell you decided to separate and isolate will come out of the other.

Each Pack is composed of:

Hardware: 

• One OB1 flow controller DUAL
• Flow sensor MFS (see also the BFS-Coriolis flow sensor for the best performance)
• Flow resistors
• Reservoirs
• Kit starter pack Luer Lock with fittings and tubing
• ESI control software (automated sequences possible)

The whole system is based on Elveflow products that will give a high precision to the system. Also, the MFS or BFS flow sensors will allow to have real time information about the flow rate and pressure of the fluids to optimize the separation process and solve any problems that may arise.

Contact our experts to answer any questions about this biosensor testing pack and how it can match your specifications!