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Cell & biology pack

Live cell perfusion pack

Liquid handling for cell-based experiments
Multiple Media perfusion

Perform stable medium perfusion and change between several solutions

Controlled shear stress

Keep shear stress under control with a wide range of flow rates available

Microfluidic Workflows Automation

No more time consuming experiments

User-friendly

All-in-one Pack – All components & software included

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Features & Benefits

Injection Pack MuxA standard Cell & Biology Pack setup uses one pumping channel to flow multiple solutions into the microfluidic chip. In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions. All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler.

 

Advantages

  1. Control both pressure & flow rate: Ideal for shear stress assays
  2. Perform fast switches between media or drugs: For imaging cell response to various media or drugs
  3. Stable & pulseless flow rate: No more coverslip expansion and cell stress
  4. Large range of flow rates: From 10nL/min to 5 mL/min
  5. Design flow injection sequences: Create complex patterns, such as oscillating flow to mimic physiological conditions
  6. Recirculation loop: Ideal for long assays
  7. Instantaneous stop flow: For controlled solution exposure experiments, such as calcium imaging

Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion.

Contact us for more information!

Perfusion flow control system

  1. Computer: Control all the parameters with our software, and automate your experiment by creating injection sequences.
  2. Pressure & flow controller: Impose a given pressure in order to create a stable and pulseless flow rate.
  3. Splitter: Multiple your reservoir’s pressure inlet from a single controller pressure output using our splitter
  4. Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles.
  5. Rotative valve | Mux Distribution: Select the injected liquid.
  6. Flow Sensor: Monitor and control the flow rate in real time.
  7. Perfusion chamber or microfluidic chip: Elveflow can help choosing a microfluidic chip fitting your cell culture requirements.

 

Microfluidic calculator to estimate the shear stress in your cell culture chamber

To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…

Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:

Learn more on dynamic cell culture using this pack.

Learn how to automate cell seeding with this pack.

Learn more on long-term medium perfusion using this pack

BIOLOGY-RELATED FIELDS OF APPLICATION:

  • Cell confinement assays
  • Dynamic single cell screening
  • Drug screening
  • Cell response to medium change
  • Live cell imaging
  • C.elegans stimulation
  • Cell culture on chip
  • Toxicity tests
  • 3D cell culture
  • Bioreactor studies
  • Stem cells assays
  • Cells entrapment

APPLICATION NOTES

Reviews

Publications

  • A. Chen et al., “Single Cell Mass Spectrometry with a Robotic Micromanipulation System for Cell Metabolite Analysis,” in IEEE Transactions on Biomedical Engineering, doi: 10.1109/TBME.2021.3093097.
  • Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells, Kevin Jahnke et al. Nature Communications (2021). DOI: 10.1038/s41467-021-24103-x
  • A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting, Yağmur Demircan Yalçın et al., Scientific Reports (2021). DOI: 10.1038/s41598-021-92647-5
  • Highly sensitive wide-range target fluorescence biosensors of high-emittance metasurfaces, Masanobu Iwanaga, Biosensors and Biolectronics (2021). DOI: 10.1016/j.bios.2021.113423
  • Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip, Jiandong Ding et al., ACS Applied Materials & Interfaces (2021). DOI: 10.1021/acsami.0c21186
  • In vitro skin model for characterization of sunscreen substantivity upon perspiration, Fatemeh Keshavarzi et al., International Journal of Cosmetic Science (2021). DOI: 10.1111/ics.12703
  • Electrokinetic sandwich assay and DNA mediated charge amplification for enhanced sensitivity and specificity, Siddharth Sourabh Sahu et al. Biosensors & Bioelectronics (2021). DOI: 10.1016/j.bios.2020.112917
  • The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves,  Chang Chen et al., MethodX (2020). DOI: 10.1016/j.mex.2020.101190
  • Cyclic on-chip bacteria separation and preconcentration, Vitaly Ryzhkov et al., Scientific Reports (2020). DOI: 1038/s41598-020-78298-y
  • Synthetic Biology Bicistronic Designs Support Gene Expression Equally Well in vitro and in vivo, Owen Koucky et al., American Journal of Undergraduate Research (2020).  DOI: 10.33697/ajur.2020.012
  • Zhu Z., Geng Y., Wang Y. (2021) Monitoring Single S. cerevisiae Cells with Multifrequency Electrical Impedance Spectroscopy in an Electrode-Integrated Microfluidic Device. In: Marchisio M.A. (eds) Computational Methods in Synthetic Biology. Methods in Molecular Biology, vol 2189. Humana, New York, NY. DOI: 10.1007/978-1-0716-0822-7_9
  • Investigating the Interaction Between Circulating Tumor Cells and Local Hydrodynamics via Experiment and Simulations
    Pepona, M., Balogh, P., Puleri, D.F. et al. . Cel. Mol. Bioeng. (2020). DOI: 10.1007/s12195-020-00656-7
  • A drug-compatible and temperature-controlled microfluidic device for live-cell imaging, Open Biology; Jul, 2016; T. Chen et al; DOI : 10.1098/rsob.160156
  • A microfluidic gradient generator to simulate the oxygen microenvironment in cancer cell culture; Microelectronic Engineering; Aug, 2018; Louise Orcheston-Findlay et el; DOI : 10.1016/j.mee.2018.04.011
  • Geometric Friction Directs Cell Migration; Physical Review Letters; Jul, 2013; Le Berre et al; DOI : 10.1103/PhysRevLett.111.198101
  • An Integrated Microfluidic Chip and Its Clinical Application for Circulating Tumor Cell Isolation and Single‐Cell Analysis; Cytometry; Oct, 2019; Mingxin Xu et al; DOI : 10.1002/cyto.a.23902
  • Mitotic Rounding Alters Cell Geometry to Ensure Efficient Bipolar Spindle Formation; Developmental Cell; Apr, 2013; Oscar M. Lancaster et al; DOI : 10.1016/j.devcel.2013.03.014

The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications.  Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.

CONTENT

OPTIONS

  • Additional pumping channels
  • Additional flow rate sensors
  • Microfluidics chips
  • Computer
  • Microscope and camera

Cell & biology webinars

Cell & biology videos

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Testimonials

  • “I appreciate rapid setup time and the fact that we can very precisely adjust the flow rates through the user-friendly interface. The dynamic and responsive engineering team was an additional benefit.”
    Dr. Caglar Elbuken, UNAM Bilkent University, Turkey
    OB1 pressure-driven flow controller user

  • “We really appreciate the stability and low response time of the flow controller, which is important when various flow conditions must be applied successively. The flow can be precisely and easily controlled thanks to a user-friendly interface.”
    Dr. Marie Frénéa-Robin, Laboratoire AMPERE Université Claude Bernard LYON 1, France
    OB1 pressure-driven flow controller user

  • “Elveflow provided an excellent solution to our problem, which was how to tightly control minute flows in multiple microfluidic channels. Elveflow’s support has been excellent and software development is continuing.”
    Dr. Robert Ward, Massey University, United States
    OB1 pressure-driven flow controller user

  • “Overall we are satisfied with the equipment and highly recommend it to anyone who is working in the microfluidics field.”
    Dr. Weiqiang Chen, Biomechanics Laboratory (IBBL) University of Michigan, United States
    OB1 pressure-driven flow controller

  • “I was suprised how quickly I was able to get things to work once I got going.”
    Jamie Stover, Research Assistant, M. Zernicka-Goetz Lab at California Institute of Technology (Caltech)
    OB1 pressure-driven flow controller user

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