contact@elveflow.com+33(0).184.163.807+1(414)-406-4343 Elveflow is an Elvesys brand, an international microfluidics innovation center with high level researchers dedicated to microfluidics and organ-on-a-chip.
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Adam Fenster / University of Rochester. Licence: Attribution 4.0 International (CC BY 4.0) Image Credit: Norovirus – colour-enhanced. David Gregory & Debbie Marshall. Licence: Attribution 4.0 International (CC BY 4.0) ) [principe] => Array ( [texte] => Biosensor testing
Image Credit: J. Adam Fenster / University of Rochester. Licence: Attribution 4.0 International (CC BY 4.0)
Image Credit: Norovirus – colour-enhanced. David Gregory & Debbie Marshall. Licence: Attribution 4.0 International (CC BY 4.0)
This beta pack is dedicated to the test, characterization, and calibration of biosensors with small volumes, reproducibility, and automatization. We have sought to create a system that unifies and automates the tests necessary to calibrate any biosensor based on Elveflow instruments.
The main objective of this pack is to create a scalable system that allows you to accurately calibrate a biosensor through microfluidic technology. With this system, you can test different solutions (drugs or reagents at different concentrations) in order to calibrate or test your biosensor in an easy way. In addition, this system can be adapted to test any fluid, liquid or gaseous in order to calibrate a biosensor for any application with a sequential fluid Injection system.
AN ALL-IN-ONE PACK TO EASILY TEST YOUR BIOSENSOR
Biosensors are analytical devices that have taken on an important role in the biotechnology sector. This is because they have a wide variety of applications and can be adjusted to different criteria of sensitivity and precision. Biosensors are responsible for converting biochemical signals into signals that can be quantified, which are typically electrical or optical signals. It is for this reason that biosensors must be properly calibrated in order to quantify and minimize the error in each one of them. For this reason, it is necessary to develop an automated system that allows performing different tests to optimize the performance of a biosensor. This is an important activity since one of the most important activities of a biosensor is to obtain accurate information in real-time about the measurements it performs. Also, because a biosensor uses different biological components, it is necessary to take into account a cleaning system between each calibration test.
While for a proof of concept, manual testing works very well, this requires a lot of pipetting time that you may want to get rid of for scalability reasons. As soon as you need more accurate professional biosensor testing, an automated system can take you to the next level ensuring better control and accuracy in calibration.
In general, microfluidics will allow you to have high precision because thanks to a microfluidic pump you can control the flow of each sample precisely. This will decrease the variability between results and increase the accuracy obtained in each of the measurements. Similarly, through a microfluidic system, you can decrease the test time by increasing the transport speed of the samples.
In addition, this microfluidic system will allow you to obtain great flexibility since it can be adapted to each biosensor you want to calibrate and at the same time to the specifications of each application. Thanks to the performance of this set of equipment, you will be able to control all the parameters that you need.
If you already want to start testing your biosensors you can explore the different configurations that this Elveflow beta pack has for you.
If you already want to start with the setup of this beta pack you can check the following examples of sensor calibration beta packs on different types of microfluidic chips and type of experiments. In general, these are based on the calibration through the injection of different types of samples thanks to the sequential injection system. For more detailed information, please contact our experts or check our multiple fluid switching application pack.
Depending on the intended application of your biosensor it is important that you choose how you want your biosensor testing system to perform. For example, this setup can be used when you want to test a biosensor by varying the input substances.
With our beta pack you will be able to test different liquids in your biosensor. However, if you then wish to vary the concentration, you can consult our beta pack for concentration calibration in biosensors.
Each Pack is composed of:
Hardware:
Contact our experts to answer any questions about this biosensor testing pack and how it can match your specifications!
To perform any experiment involving a biosensor it is important to first test its performance. This activity will allow you to determine how sensitive and reliable your sensor will be to different samples. To determine the functionality of your biosensor it is necessary to determine some parameters such as:
Once you have decided which parameters are most important for your research you can move on to using your microfluidic device along with a customized Elvwflow pack. All these parameters can be tested easily and automatically through our all-in-one kit. Normally, the main applications of this beta pack are based on the characterization of different samples that can be further enhanced thanks to the MUX distribution.
For example, you can place up to four biosensors (one biosensor for each channel) thanks to our OB1 pressure controller. It is important to mention that thanks to the high customization provided by this beta pack, these biosensors can be different or the same so that you can ensure the repeatability of your testing. Then, you can join up to 12 samples of different sizes and administer them sequentially to your biosensors.
This way, you can have automated testing of your biosensors in simple steps, compared to using a conventional system that will only allow you to test one sample on a biosensor per step. Therefore, with the biosensor testing pack you will optimize the testing time as well as the amount of samples you need. Microfluidic testing only requires tiny volumes of samples or reagents, so you can easily run different tests and automate them with each other.
It is important to note that because biosensors treat biological samples, it is necessary to have a buffer cleaning step between each test. This process generates more accurate and closer to reality results; however, it increases the testing process if a conventional calibration method is used.
For that reason, with the Elveflow beta pack you can save processing time as the buffer cleaning process can be easily included in the pack.
One of the most common applications for biosensors is based on the variation of the type of drug that these sensors can measure. Thanks to the beta test pack you can vary the type of drug to be tested to evaluate which one has a better performance for your experiment. Also, at the same time you can decide whether to test the performance of different biosensors as you can add up to 4 biosensors for simultaneous testing. The variation of drugs in experimentation has a high relevance and needs an accurate testing system like the one offered by this beta pack since this type of system can be used for the detection of drugs in biological fluids such as sweat, saliva, blood or urine. In addition, it can be used to ensure safety in the quality control process in drug production or in the food industry.
If you wish to go further with the testing of your biosensor you can consult our MUX cross chip and contact our experts who will help you determine which beta pack fits your needs.
Yes, parallelization can be achieved with this beta pack and will allow you to simultaneously analyze multiple samples or detect multiple analytes. By incorporating multiple samples a biosensor can increase its performance and efficiency, making it suitable for multiplexed analysis or simultaneous monitoring. This will allow you to have faster analysis of multiple samples, reduce the time required for data collection, and improve the overall sensitivity and specificity of the biosensor.
With our system it will be easier for you to obtain data so that you can obtain a calibration curve for your biosensor. Depending on the intended use of your biosensor, you can apply different types of configurations. For instance, if you want to test multiple sensors at the same time, you can add a manifold to distribute the inlet fluid to all the biosensors. Additionally, it is possible to add a valve to maximize the use of each pressure channel.
Moreover, it is possible to integrate a mixing chip before biosensor testing to perform concentration tests with different substances, as the chip will allow the fluids you choose to combine properly.
Furthermore, it is possible to add an external buffer substance input to have better control over the cleaning of each part of the system between each test.
Yes, this beta pack can be used together with any other Elveflow equipment since they are all compatible with each other. This feature allows you to adapt this pack to your needs.
Sterilization in biosensor testing is important to ensure the accuracy, reliability and safety of the tests you perform. In that sense, having good contaminant management can help you ensure reproducibility and comparability of results. For that reason, this beta pack considers the buffer cleaning steps in each test cycle of your biosensors. However, for specific applications just contact our expert to check if your method is suitable with our instruments or follow the protocol they will share with you.
Elveflow equipment is ready for you to use in a wide range of volumes, ranging from microliters to liters. However, to find out which Elveflow equipment and features match your applications, contact our experts.
Elveflow’s products and Beta packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
Elveflow can provide you with a wide range of different flow sensors, bubble detectors and traps or any relevant microfluidic instrument for the applications you need.
Please contact our experts for any questions about this pack and how it can fit your specifications.
Elveflow® provides a perfusion system dedicated to cell culture, for lab-on-chips, flow cells and perfusion chambers. This pack includes all the necessary elements to create a continuous flow and monitor flow rate applied on the cells.
Ideal for experiments requiring switches between different cell culture media. A computer-controlled valve allows sequential injections (up to 12 different medium or reagents, more on demand).
The intuitive ESI control software allows to quickly automate your complex experimental workflows.
A standard Cell & Biology Pack setup uses one pumping channel to flow multiple solutions into the microfluidic chip. In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions. All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler.
Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion.
Contact us for more information!
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:
Learn more on dynamic cell culture using this pack.
Learn how to automate cell seeding with this pack.
Learn more on long-term medium perfusion using this pack
The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications. Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.
An all-in-one flow control system for fast switching of fluids while maintaining a defined flow rate.
Several experiments or tests can be carried out with one system at once so as to automate your multiple liquid injection / testing and save time!
Elveflow’s sequential fluid injection pack is dedicated to any system that requires to quickly swap between several fluids while maintaining a precise flow rate. This makes it a perfect fit for your complex organic chemistry reactions, organic synthesis, biosensors, biochemical sensors or electrochemical sensors, test rigs, flow chemistry, Seq-Fish experiment, analytical chemistry setup, drug and toxicity testing, particle synthesis, organocatalysis experiment, mother machine for antibiotic resistance testing and many more…
This pack is suitable for both biological and chemical experiments such as multiple perfusion switch of liquid media and automated sequential reagent injection.
This pack includes all the necessary elements to sequentially inject up to 12 (or more on request) solutions in a fully automated fashion using our computer-controlled 12 to 1 MUX Distribution bidirectional valve, high accuracy OB1 flow controller, and intuitive ESI control software.
A large flow rate range (from 7 nL/min to 30+ mL/min) and volumes (100 µL to up to several Liters) are accessible with this system.
The typical Sequential fluid injection pack setup uses only one single pressure channel to sequentially flow multiple solutions into your microfluidic system. Medium change can then easily be carried out using the MUX Distribution‘s rotary valve that acts as a liquid selector and allows fast switching and selection between your solutions.
In combination with an MFS or BFS flow sensors, the OB1 flow controller allows an extremely stable liquid perfusion (down to 0.006% of the flow rate).
All these operations can be performed using the ESI software interface. The software allows you to fine-tune the flow parameters as well as to automate your experiment using our intuitive scheduler.
More information on how to perform a stable and precise flow switch in our example application note.
Our sequential fluid injection pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Contact us to know more!
To discover more tips and tricks…
Claim our complete User guide about Automated Fluid Sequential Injection!
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The sequential fluid injection pack can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
For more biology-related content, please refer to the cell perfusion application pack.
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et al., Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K
Microfluidic platform for 3D cell culture with live imaging and clone retrieval
C. Mulas et al., Lab on a chip, 2020, 20, 2580-2591, DOI: 10.1039/D0LC00165A
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the Sequential fluid injection Pack can be adjusted to suit your specific needs. Contact us for more details.
At small scale, liquid handling can be controlled precisely with the help of microfluidics.
This all-in-one system for the sequential injection of liquids/medium is based on two centerpieces: a pressure controller and a rotary valve.
The pressure-driven flow controller allows higher stability, better response time compared to systems using a peristaltic pump or a syringe pump,
This liquid handling pack is simple to set-up and to operate and enables easy system automation.
The platform can be adapted to any requirements and fully automated.
Our liquid handling pack enables to sequentially inject different liquids. It contains: a MUX Distribution bidirectional valve which works as a selector, a high accuracy flow controller, and Elveflow’s intuitive ESI control software.
The pack also contains a manifold to pressurize several reservoirs using a single pressure channel from the OB1 and a flow sensor to monitor and control the flow rate in real-time.
The MUX distribution rotary valve is characterized by:
If your experiment requires the use of a buffer in large quantities but also expensive reagents in tiny volumes, it is not an issue for our liquid handling pack. You can actually connect tanks with various volumes, from bottle to Eppendorf.
Moreover, there is no restriction concerning the use of liquids as the wetted materials of the system are resistant to aggressive reagents. The flow sensors can also be chosen to work with liquids of different properties.
Our liquid handling pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Get in touch to claim our complete Userguide about Liquid handling & Sequential Injection!
The liquid handling pack can be used in various fields of application and for any experiment that require successive injection of liquids:
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the liquid handling pack can be adjusted to suit your specific needs. Contact us for more details.
Selecting the best flow control method for your application is very important, since the performance and fluid handling efficiency of a microfluidic device depends highly on the flow control system. If you require to use our pack for the previously mentioned applications, the solution will contain:
You can choose from the following additional options:
Credit: Red blood cells forming rouleaux. Jonathan Armstrong. Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Viscosity is a very important parameter in many different types of research and applications. For that reason, parameter accuracy and parameter control is important. Microfluidics is one of the most valuable methods to measure viscosity for the following reasons:
If you already want to start testing your biosensors you can explore the different configurations that this use case has for you.
The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guaranties the best measurements.
Put different types of measure of viscosity
Elveflow’s products and packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
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Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications. ) [insert] => ooc2 [utiliser_acf] => [most_popular] => [review_type] => 13 [citation_author_repeater] => [citation_publication_date] => 2023/08/14 [citation_keywords_repeater] => [description_GS] => [header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 3673 [title] => 3D Cell Culture Methods and Applications [permalink] => https://elveflow.com/microfluidic-reviews/3d-cell-culture-methods-and-applications-a-short-review/ [post_type] => reviews [post_type_name] => Review ) [11] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 593 [id] => 593 [title] => free copyright microfluidic researcher [filename] => free-copyright-microfluidic-researcher.jpg [filesize] => 74971 [url] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [link] => https://elveflow.com/microfluidic-reviews/researchers-opinion-on-flow-control-for-microfluidics/free-copyright-microfluidic-researcher/ [alt] => free copyright microfluidic researcher [author] => 27 [description] => free copyright microfluidic researcher [caption] => free copyright microfluidic researcher [name] => free-copyright-microfluidic-researcher [status] => inherit [uploaded_to] => 592 [date] => 2019-08-28 14:40:09 [modified] => 2024-08-19 14:21:17 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://elveflow.com/wp-includes/images/media/default.png [width] => 1280 [height] => 960 [sizes] => Array ( [thumbnail] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-300x225.jpg [medium-width] => 300 [medium-height] => 225 [medium_large] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-768x576.jpg [medium_large-width] => 768 [medium_large-height] => 576 [large] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-1024x768.jpg [large-width] => 1024 [large-height] => 768 [1536x1536] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [1536x1536-width] => 1280 [1536x1536-height] => 960 [2048x2048] => https://elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [2048x2048-width] => 1280 [2048x2048-height] => 960 ) ) [texte] => One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip. ) [utiliser_acf] => [most_popular] => [review_type] => 9 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => flow control ) ) [description_GS] => One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip. 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However, controllable and fast mixing is critical for microfluidic and lab-on-chip devices. 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Moreover, miniaturization and integration of the multiple assay components permit automation, precise flow control, increased reproducibility, and the possibility for high-throughput analysis. ) [insert] => starter2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Saif Mohammad Ishraq Bari ) [1] => Array ( [citation_author] => Gergana G. Nestorova ) ) [citation_publication_date] => 2021/01/25 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => Thermoelectric sensor ) ) [description_GS] => Introduction to thermoelectric sensor | Microfluidics immunosensors offer multiple advantages over the conventional immunoassays... 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This review focuses on point of care (POC) diagnostic devices for pathogen detection
Microfluidics is a term which appears more and more often in papers and scientific magazines; but, what exactly is microfluidics?
Microfluidic flow cells with embedded optical sensors, have been the most common choice for microenvironment monitoring like pH control.
Lab-On-a-Chip drug testing in Microfluidics
Centrifugal microfluidics, or "Lab-on-a-CD," leverages centrifugal force to manipulate fluids on a microscale.
The integration of CRISPR-Cas9 with microfluidics has led to the development of innovative techniques for genetic editing and screening.
There are three main classes of systems to control liquid motion in microfluidic and nanofluidic devices.
A lab-on-a-chip is a miniaturized device that integrates onto a single chip one or several analyses, which are usually done in a laboratory; analyses such as DNA sequencing or biochemical detection.
One of the key criteria to choose your microfluidic device material is its chemical resistance. This review will help you choose one depending on your application.
Electrochemical detection is particularly suitable for lab-on-a-chip integration and microfluidics due to the versatility of size, geometry and nature of electrodes that can be integrated within a microfluidic platform, and to the minimum instrumentation it requires.
Cell culture consists in growing cells in an artificial environment in order to study their behavior in response to their environment[1]. Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications.
One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip.
In microfluidic laminar flows, traditional turbulent mixing between two liquids cannot occur. However, controllable and fast mixing is critical for microfluidic and lab-on-chip devices. So different mixing techniques were developed and are here presented.
Microfluidics involve different types of devices, and materials for microfluidic fabrication must be selected while bearing in mind all the requirements necessary for building a fine microfluidic device.
Suitable detection techniques are required to be coupled to microfluidic technology in order to analyze experiment outcomes in a sensitive and scalable way.
In this review, we will present the PCR, qPCR and other associated methods with their microfluidic applications.
Today, MICROFLUIDICS is a distinct and major technological field, but 20 years ago it was not like this and its boundaries were not so well defined. The history of microfluidics is strictly related to several other areas
Introduction to thermoelectric sensor | Microfluidics immunosensors offer multiple advantages over the conventional immunoassays that include improved reaction rate, reduced time for incubation of the reactants, and decreased reagents and sample consumption. Moreover, miniaturization and integration of the multiple assay components permit automation, precise flow control, increased reproducibility, and the possibility for high-throughput analysis.
Fluid mixing at microscale is of importance for many fields of application. This short review summarises the key takeaway aspects of fluid mixing at microscale.
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Discover the innovative use of elastin-like polypeptide (ELP) coacervates as reversibly triggerable compartments in synthetic cells. Explore how phase separation, dynamic compartmentalization, and lab-on-a-chip techniques redefine synthetic cell engineering.
This short review explores the fabrication of a leak resistant microfluidic chip. Most importantly, the 4 channel PDMS-based microfluidic chip is also tested under pressurized fluid injection for multiple liquid leak resistance checks.
Electrokinetic sandwich assay and DNA mediated charge amplification
Complex droplet networks using pressure-driven microfluidics
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Active droplet generation in microfluidics is of high interest for a wide range of applications. It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. This additional control is extremely desirable for complex operations which rely on the accurate control of both parameters.
This application note explains how to set up a robust and reproducible microfluidic platform for liposomes assembly with improved encapsulation efficiency and reduced polydispersity in size.
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Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers. 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[thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://elveflow.com/wp-content/uploads/2024/11/OB1-shcematics-300x230.gif [medium-width] => 300 [medium-height] => 230 [medium_large] => https://elveflow.com/wp-content/uploads/2024/11/OB1-shcematics-768x588.gif [medium_large-width] => 768 [medium_large-height] => 588 [large] => https://elveflow.com/wp-content/uploads/2024/11/OB1-shcematics.gif [large-width] => 912 [large-height] => 698 [1536x1536] => https://elveflow.com/wp-content/uploads/2024/11/OB1-shcematics.gif [1536x1536-width] => 912 [1536x1536-height] => 698 [2048x2048] => https://elveflow.com/wp-content/uploads/2024/11/OB1-shcematics.gif [2048x2048-width] => 912 [2048x2048-height] => 698 ) ) ) [introduction_texte] => ) [principe] => Array ( [texte] => What is a microfluidic pressure-driven flow controller? <br /> Cutting-edge Microfluidic Flow Controller Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs. Check out our OB1 animation here! The best performance on the market: Piezoelectric Technology The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market. Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want. Get the fastest flow rate control when paired with a flow sensor Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate. Full control software, SDK, and UART communication Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC. OEM version available The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution. [image] => ) [applications] => Array ( [texte] => Applications of Pressure-Driven Flow Control Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains. Droplet & Bead Generation Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack. Biology & Cell Culture Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study Flow Control & Mixin Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup Analytical Chemistry & Imaging Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC Industrial Applications Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review Cosmetic Formulation & Testing Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications Integrated Systems Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review Videos Publications For all publications featuring our products, please click here. For all application notes based on our product, please click here. For reviews of our product, please click here. ) [specifications] => Array ( [texte] => This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller. OB1 MK4 CHANNEL PRESSURE RANGE 0 to 200 mbar1 (0 to 2.9 psi) 0 to 2,000 mbar1 (0 to 29 psi) 0 to 8,000 mbar1 (0 to 116 psi) -900 to 1,000 mbar1 (-13 to 14.5 psi) -900 to 6,000 mbar1 (-13 to 87 psi) Pressure stability (2) 0.015% FS 30 µbar (0.0004 psi) 0.005% FS 100 µbar (0.0014 psi) 0.006% FS 500 µbar (0.007 psi) -900 to 500 mbar: 0.005% FS 100 µbar (0.0014 psi) 500 to 1,000 mbar: 0.007% FS 150 µbar (0.0021 psi) -900 to 2,000 mbar: 0.005% FS 350 µbar (0.005 psi) 2,000 to 6,000 mbar: 0.007% FS 525 µbar (0.008 psi) Response time (3) down to 10 ms Settling time (4) down to 50 ms Minimum pressure increment 0.006% FS 12 µbar – 0.00017 psi 0.006% FS 120 µbar – 0.0017 psi 0.006% FS 480 µbar – 0.007 psi 0.0064% FS 120 µbar – 0.0017 psi 0.0061% FS 420 µbar – 0.006 psi Pressure supply 1.5 bar to 10 bar Non corrosive, non explosive, dry and oil-free gases, e.g., air, argon, N2, CO2, … Input vacuum (5) / / / Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Liquid compatibility Non contact pump Any aqueous, oil, or biological sample solution. Non-contractual information, may be changed without notice (1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only Flow control Flow sensor compatibility Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available Flow rates From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series) Liquid compatibility Non-contact pump Any aqueous, oil, or biological sample solution. Control & monitoring Software control Elveflow Smart Interface – Windows 7, 8, 10, both 32 and 64 bit versions supported Software Development Kit Libraries available: Matlab, Python, LabView, C++ – Windows 7 & 10, both 32 & 64 bit versions supported Serial/UART communication protocol on request Data management Possibility to log and extract data (CSV): channel and sensor detailed information using ESI Input profiles Possibility to load profiles: ramp, sine, triangle, square, or custom Automation Generate step-by-step sequences using the ESI built-in sequence management Log and export custom configurations (CSV) Screen LCD screen showing pressure and sensor flow rate in real time Electrical Specifications Input Voltage (V) 24V Typical Power (W) 12W Provided Power Supply Specifications Supply Voltage Range (V): 100 to 240 VAC Supply AC Frequency (Hz): 50 to 60 Hz Maximum Output Current (A): 1.5 A Maximum Output Power (W): 36W Interface USB Type B Communication Type Serial Software Control ESI Sensor Connection One M8 4-pins connector per channel Compatibility Elveflow sensors: MFS, MPS, MFP, MBD Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage Triggers Input and Output TTL signal 0V or 5V Other Casing dimensions (length x width x height): 240 x 223 x 80 mm Weight 1.4 kg to 2.9 kg Non-contractual information, may be changed without notice [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => Array ( [0] => Array ( [question] => What pressure range should I choose? [reponse] => The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range. In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures. ) [1] => Array ( [question] => Can I achieve direct flow control? [reponse] => By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value. The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor). ) [2] => Array ( [question] => Can I connect a Pressure Sensor to the OB1? - [reponse] => Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave). ) [3] => Array ( [question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ? [reponse] => Yes, you will need a pressure source to work with the OB1 pressure controller. You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1. If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1. ) [4] => Array ( [question] => How to install the OB1? [reponse] => Click here Unboxing video ) ) ) [more] => Array ( [more_title] => Software [texte] => Software ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code. The OB1 control window allows to:
The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications.
Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers.
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.
Check out our OB1 animation here!
The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want.
Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.
Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.
The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution.
Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.
Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup
Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.
Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup
Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems
Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example
Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example
Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study
Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack
Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup
Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application
Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research
Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC
Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review
Cosmetic Formulation & Testing
Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications
Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review
For all publications featuring our products, please click here.
For all application notes based on our product, please click here.
For reviews of our product, please click here.
This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller.
Non-contractual information, may be changed without notice
(1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
Click here
Unboxing video
ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.
The OB1 control window allows to:
For more details about Pressure driven flow control, please read this application note.
By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision.
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
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