Beta Pack

Organoids and spheroids are three-dimensional structures composed of self-assembled cells that mimic the characteristics of tissues and organs. These structures are typically grown by culturing cells under non-adherent conditions that allow them to self-organize into these complex structures [1]. A current challenge is to integrate cellular complexity into organoids in a controlled manner, leading to organized assembly and takeover of tissue function [2].

Using microfluidics for organoids or spheroids in droplets:

• provides an environment with different physicochemical properties, such as stiffness or permeability, which can influence organoid formation and function
• allows a wide variety of polymers to be be used for the dispersed phase is ideal for generating high throughput and reproducible organoids or spheroids the precision and composition of droplets, which are critical for uniform and reproducible organoid formation

Elveflow offers you a versatile computer-controlled microfluidic setup to enable organoid & spheroid encapsulation in an efficient manner.

ORGANOID & SPHEROID ENCAPSULATION PACK: THE PRINCIPLE

One technique for creating organoids and spheroids is to encapsulate cells in droplets of a hydrogel, such as alginate or agarose, using microfluidic devices. The droplets act as small bioreactors, providing the cells with the necessary nutrients and oxygen for growth, while also allowing for the exchange of waste products.

ORGANOID & SPHEROID ENCAPSULATION PACK: THE SETUP

This pack is composed of the premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment set, you will be able to:

• produce robust and highly monodisperse emulsions (CV<3%)
• Fast and efficient mixing of reagents for droplet generation
• have access to all the necessary elements to encapsulate cells easily
• provide ultra-precise pressure-driven flow control
• generate 10 to 80 µm diameter droplets (and more depending on your microchip)

The solution can be configured as per the user’s requirements. Our experts can help you define the best possible setup to reach your goal.  

Thanks to the versatility of the instruments, and the upgradability of our range, Elveflow’s solution can be adapted to your needs.

A TYPICAL PACK CONTAINS:

• OB1 flow controller with 2 control channels (one for each phase)
• Two flow rate sensors (one for each channel)
• Two sets of flow resistors (for stable and accurate flow control)
• Two Microchips from ChipShop, a leading microfluidic chip manufacturer.
• All necessary reservoirs and tubings.
• Automation and control software
• A user guide

As proof of concept, you can consult this Nature paper on Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response

References

[1] Kratochvil, M. J., Seymour, A. J., Li, T. L., Paşca, S. P., Kuo, C. J., & Heilshorn, S. C. (2019). Engineered materials for organoid systems. Nature Reviews Materials, 4(9), 606-622.

[2] Takebe, T., & Wells, J. M. (2019). Organoids by design. Science, 364(6444), 956-959.