Cell confinement pack for biological applications

Cell confinement Pack

Cell compression for mechanical cell stimulation experiments

Mechanobiological cell response

Allows advanced molecular and morphological analysis of confined cells

Precise mechanical stimulus

Trigger mechanical stimulus by adjusting confinement in real time

Standard cell culture plate

Compatible with 35 mm petri dishes and flat surfaces

Easy to use

Fast experiment set-up with plug-and-play equipment

We combined the Elveflow pressure control technology with the state-of-the art 4D Cell Confiner to create the ideal experiment conditions for biomechanical analyses of cells and tissues.

Features & Benefits

The Mechanobiology pack brings together 4Dcell‘s cell confinement technology with the best in pressure control from Elveflow.

A standard setup contains a pressure controller and a cell confiner to better mimic in vivo cell culture conditions. The OB1 pressure controller from Elveflow generates stable pressure and vacuum while the cell confiner from 4D Cell provides micrometric control of the culture microenvironment.

The combination of cell compression and spatial control provides insight into cell behavior in confined spaces and under mechanical stress, increasing the translational value of in vitro cell-based assays.

Advantages for mechanobiology studies

Control both pressure & physical space: Ideal for mechanical stress assays.
Allows fast switches between pressure settings: For real-time observation of cell responses to mechanical stimulus.
Stable & pulseless pressure: Total and stable control over cell compression.
Non-destructive cell assay: Retrieve your cells for molecular analysis.
Instantaneous start and stop pressure: Rapid trigger and release confinement.

Our dedicated pack can be adapted for more complex and advanced experiments. Contact us for more information!

Contents of the Cell confinement pack

1 x OB1 pressure controller
1 x Pressurized reservoir
3 x 4Dcell Confiner (PDMS suction cup)
- PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinment is applied
12 x Confinement slides:
- 10 mm diameter glass cover slips containing the PDMS micro-structures (pillars)
- Confinement heights from 1 to 20 µm (you may select up to 3 heights for your kit)
1 x Tubing and connectors kit

BIOLOGY-RELATED FIELDS OF APPLICATION:

Cell confinement assays
Dynamic cell behavior
Cell migration and cancer metastases
Mechanotransduction
Cell migration plasticity
Cell response to mechanical stress
Live cell imaging
Cell division
Mechanical-induced cell repair
Genomic instability during cell division
Wound healing and inflammation studies
Cell entrapment

Reviews:

The amazing benefits of microfluidics can be applied to many cell & biology perfusion experiments and applications. Therefore, the content of the pack can be adjusted to suit your specific needs. Contact us for more details.

CONTENT

1 x OB1 pressure controller
1 x Pressurized reservoir
3 x 4Dcell Confiner (PDMS suction cup)
- PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinement is applied
12 x Confinement slides:
- 10 mm diameter glass cover slips containing the PDMS micro-structures (pillars)
- Confinement heights from 1 to 20 µm (you may select up to 3 heights for your kit)
1 x Tubing and connectors kit
3 x PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinement is applied

CONFINEMENT PROTOCOLE

The system is assembled by connecting the suction cup to a tube and the pressure controller, initially set to -3 kPa. The confinement slide is carefully placed onto the suction cup with the pillars facing upward.
To activate confinement, the device is brought into contact with the cell culture substrate and the pressure is reduced to -10 kPa, which sets the confinement. Finally, to release it, the pressure is returned to -3 kPa, allowing the device to detach.
for more details you can have a look on 4DCell dynamic confiner page