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OB1 MK4

Microfluidic Flow Controller

1 to 4 channels pressure & vacuum microfluidic flow controller
PRESSURE AND VACUUM CONTROL

Accurately control pressure to push and pull liquid

FLOW CONTROL

Pair it with a flow sensor for accurate flow control

UNMATCHED PERFORMANCE

High precision and responsiveness for your flow regulation

MICROFLUIDIC AUTOMATION

Automate and control your microfluidic experiment

Talk to an expert GitHub Documentation

Features & Benefits



What is a microfluidic pressure-driven flow controller?

Cutting-edge Microfluidic Flow Controller

Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications.
Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.


OB1-schematics

Check out our OB1 animation
here!





The best performance on the market: Piezoelectric Technology

The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.


Piezoelectric technology




OB1 microfluidics flow controller pressure outlets elveflow

Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available

The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options).
If your needs change, the instrument can be upgraded later, in any way you want.




Get the fastest flow rate control when
paired with a flow sensor

Connect the OB1 MK4 to a standard liquid flow rate sensor
(MFS)
or our premium Coriolis flow sensor
(BFS, suitable for both liquid and gas)
to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.


BFS flow sensor microfluidics




Sensor reading unit for Elveflow products with software

Full control software, SDK, and UART communication

Single and intuitive software to get started in a few clicks and automate complex and long experiments.
The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments.
The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.




OEM version available

The OB1 MK4 can be used on a bench setup or embedded in your own product.
Elveflow has a solution for every step of your research & development.
Discover our
OB1 MK4 OEM solution.


OEM-fluidic-components-product-page

Applications of Pressure-Driven Flow Control

Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.

 

Droplet & Bead Generation

Droplet Microfluidics
Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup

Alginate Bead Generation
In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.

 

Biology & Cell Culture

Cell Perfusion
Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup

Organ-on-a-Chip
Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems

Cell Confinement Assays
Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example

Cell Constriction Assays
Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example

Cell Trapping & Synthetic Compartments
Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study

 

Flow Control & Mixin

Sequential Injection & Mixing
Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack

Recirculation
Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup

 

Analytical Chemistry & Imaging

Flow Chemistry
Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application

Liquid-Phase Electron Microscopy (LPEM)
Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research

Light-Field Flow Cytometry (LFC)
In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC

 

Industrial Applications

Enhanced Oil Recovery (EOR)
Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review

Cosmetic Formulation & Testing

Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications

 

Integrated Systems

Lab-on-a-Chip Platforms
At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review

 

Videos

Publications

For all publications featuring our products, please click here.

For all application notes based on our product, please click here.

For reviews of our product, please click here.

This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller.

OB1 MK4 CHANNEL PRESSURE RANGE 0 to 200 mbar1
(0 to 2.9 psi)
0 to 2,000 mbar1
(0 to 29 psi)
0 to 8,000 mbar1
(0 to 116 psi)
-900 to 1,000 mbar1
(-13 to 14.5 psi)
-900 to 6,000 mbar1
(-13 to 87 psi)
Pressure stability (2) 0.015% FS
30 µbar (0.0004 psi)
0.005% FS
100 µbar (0.0014 psi)
0.006% FS
500 µbar (0.007 psi)
-900 to 500 mbar:
0.005% FS
100 µbar (0.0014 psi)
500 to 1,000 mbar:
0.007% FS
150 µbar (0.0021 psi)
-900 to 2,000 mbar:
0.005% FS
350 µbar (0.005 psi)
2,000 to 6,000 mbar:
0.007% FS
525 µbar (0.008 psi)
Response time (3) down to 10 ms
Settling time (4) down to 50 ms
Minimum pressure increment 0.006% FS
12 µbar – 0.00017 psi
0.006% FS
120 µbar – 0.0017 psi
0.006% FS
480 µbar – 0.007 psi
0.0064% FS
120 µbar – 0.0017 psi
0.0061% FS
420 µbar – 0.006 psi
Pressure supply 1.5 bar to 10 bar
Non corrosive, non explosive, dry and oil-free gases, e.g., air, argon, N2, CO2, …
Input vacuum (5) / / / Any value from -0.7 to -1 bar
Compatible with vacuum pump or vacuum line
Any value from -0.7 to -1 bar
Compatible with vacuum pump or vacuum line
Liquid compatibility Non contact pump
Any aqueous, oil, or biological sample solution.

Non-contractual information, may be changed without notice


(1) Max pressure value might vary by +/- 2.5%
(2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150)
(3) Time required to reach 5% of the setting point. Depends on the computer operating system
(4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar
(5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only

Flow control
Flow sensor compatibility Compatible with the whole MFS and BFS range
Monitoring and feedback loop flow control available
Flow rates From 0,1 µL/min to 500 mL/min
(indicative, please refer to the MFS and BFS series)
Liquid compatibility Non-contact pump
Any aqueous, oil, or biological sample solution.
Control & monitoring
Software control Elveflow Smart Interface – Windows 7, 8, 10, both 32 and 64 bit versions supported
Software Development Kit Libraries available: Matlab, Python, LabView, C++ – Windows 7 & 10, both 32 & 64 bit versions supported
Serial/UART communication protocol on request
Data management Possibility to log and extract data (CSV): channel and sensor detailed information using ESI
Input profiles Possibility to load profiles: ramp, sine, triangle, square, or custom
Automation Generate step-by-step sequences using the ESI built-in sequence management
Log and export custom configurations (CSV)
Screen LCD screen showing pressure and sensor flow rate in real time

 

Electrical Specifications
Input Voltage (V) 24V
Typical Power (W) 12W
Provided Power Supply Specifications Supply Voltage Range (V): 100 to 240 VAC
Supply AC Frequency (Hz): 50 to 60 Hz
Maximum Output Current (A): 1.5 A
Maximum Output Power (W): 36W
Interface USB Type B
Communication Type Serial
Software Control ESI
Sensor Connection One M8 4-pins connector per channel
Compatibility Elveflow sensors: MFS, MPS, MFP, MBD
Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage
Triggers Input and Output TTL signal 0V or 5V
Other
Casing dimensions (length x width x height): 240 x 223 x 80 mm
Weight 1.4 kg to 2.9 kg

 

Non-contractual information, may be changed without notice





The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.


In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.










By coupling a MFS or BFS flow sensor to the  OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.


The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).










Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).









Yes, you will need a pressure source to work with the OB1 pressure controller.


You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.


If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.






Software

ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.

The OB1 control window allows to:

  • Visualize the key parameters of each channel:
    • the command pressure
    • the output pressure
  • Visualize sensor output when one is connected to the OB1  (a flow rate sensor on the first channel in the video presented below)
  • Set any pressure or flow rate pattern (predefined or custom)
  • Easily switch to the flow rate control mode (sensor mode)
  • Use the pause/play button to preset the parameters on each channel and launch them simultaneously
  • Save configurations to gain time in the future or automate your workflow thanks to the sequencer
  • Open a graph to visualize and save data
  • Launch and use dedicated modules to simplify specific experimental tasks

 

 

Which sensor is compatible with OB1 MK4?

How to choose your device-OB1 MK4

colored bubble

Testimonials

Advantages of Pressure control in microfluidics

  • Fast settling time
  • High stability and pulseless flow
  • Possibility to handle fluid volumes of several liters
  • Enable both flow and pressure control when used with a flow meter

For more details about Pressure driven flow control, please read this application note.

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