Setup : OB1 MKIII pressure controller + MUX Inj + Flow Sensor + Aline Inc Organ on a Chip

How to perform fluid recirculation?

The MUX inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two set-up configurations. One application is to make a recirculating set-up with a flow rate always going through the chip in the same direction. In this application note, we walk you through the steps of setting up a unidirectional recirculation through a semi permeable membrane.

UNIDIRECTIONAL SHEAR STRESS

Apply a unidirectional shear stress over your chip

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Mux Inj working principle

The injection loop consists of a network of inputs/outputs that can be connected to each other in two configurations. Its very low internal volume (Stator- 0.2 μL/hole, Rotor Seal- 1.3 μL/groove) makes it the ideal valve to perform the injection of a controlled volume of sample in a chip.

The Mux Inj has 6 inputs and two configurations shown on the image below. Each input is connected to a neighbour output, either on the right or the left depending of the configuration.

Configuration 1

Configuration 2

Applications

  • Cell culture on chip
  • Live cell imaging
  • Cell response to medium change
  • Drug screening
  • Toxicity tests
  • Stem cells assays

Set-up

Material

Organ on a chip (Aline Inc.)

recirculating-perfusion-microfluidic-system2

Elveflow® pressure & flow control instrument (OB1 MK3)
MUX inj
Sample reservoirs (a Small Reservoir, Medium Reservoir or a Large Microfluidic Reservoir), one for each medium sample
Microfluidic device (here an Organ on a chip microfluidic chip from Aline Inc…)

We are using here an organ on a chip to show case the recirecirculating-perfusion-microfluidic-system2rculating setup.

This chip, from Aline Inc, is a two input two outputchips. It consists of two channels, one upper channel and one above channel, separated by a semi permeable membrane. The idea of the experiment is to have a continuous and constant flow of for instance cell nutriment in the upper channel. This nutriment is recirculating during a given period of time. In the below channel, there is a continuous flow of for instance cells, which is not a recirculating flow.

This experiment showcases how the nutriment diffuses through the membrane and which amount of the initial quantity of nutriment has been consumed by the cells. We are here using a blue dye to mimic the nutriment.

Set-up diagram

AI SCKETCH ANIM3

Warning : Make sure that the tubes are correctly inserted into the Mux inj to prevent any leakage. The tube should reach the bottom of the connector in order to perform a waterproof connection. After inserting the tubes, tight the spanner to ensure the sealing.

Step-by- step control with the Elveflow® smart interface

Conclusion

The Elveflow® smart interface allows to synchronize the OB1 MKIII pressure controller together with the Mux Inj in order to perform a recirculating set-up. This software is thought to run long term experiment thanks to the powerful sequencer which can perform the switch between reservoirs automatically either after a predetermined period of time or, in combination with a flow sensor, after a chosen amount of sample as gone through the chips.

Related tutorials

How to perform switch with a Mux Distributor and to control the flow during these switches ?

Pillarcell-microfluidic-system-for-stem-cell-differentiation-300x2377dq

How to perform cell culture on a chip ?

Pillarcell-microfluidic-system-for-stem-cell-differentiation-S

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