Microfluidic cardiac cell culture model

SET-UP TIME : 10-15 MIN / DIFFICULTY : *****

Abstract

This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller. In order to recreate a living tissue conditions, the heart cells situated insides the chips are obtained via 3D cell culture.

The OB1 Smart Interface allows to easily configure complex functions (as sinus, square, constant, etc) and add them to a project scheduler in order to mimic the numerous physiological effects of a beating heart (pressure, strain, and shear stress), subjecting cells in culture to in vivo-like conditions essential to gene expression, growth, and differentiation.

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Your needs

Possible Applications:

- Regenerative medicine/Stem cells.

- Drug discovery and testing.

- Study of other cell types.

Cell biology experiments with cell under continuous perfusion can be performed with our dedicated Perfusion Pack.

Component list

You can find each of these elements in the following gallery:

PERFUSION PACK

FOR CELL CULTURE

Microfluidic perfusion pack for cell culture

This fully integrated solution includes all the necessary elements to easily perform cell biology on chip experiments

PERFUSION PACK

The Perfusion Pack has been designed to fit most common cell biology researchers needs. Whatever you need, feel free to contact us to discuss about your exact need and if necessary we will adapt the pack and chips to your particular research application.

  • Medium renewal without any pulsation
  • Flow rate: from 0.1 µL/min to 5 mL/min
  • Easily inject drugs or reagents
  • Compatible with all kinds of slides or perfusion chambers

Piezo electric microfluidics flow control

Setup diagram

This picture shows the microfluidic setup that will be used for this application note.

Microfluidic cardiac cell culture model setup

 

As often as possible avoid using soft tubing (like Tygon) which involves compliance and then increases the response time of the system. If you’re not familiar with microfluidic tubings, you may read our dedicated tutorials.

The following diagram illustrates the complete chain of elements involved in this application note. You will need to assemble all these parts to set up your experiment:

Microfluidic cardiac cell culture model diagram

Protocol

We are going to show you an example of how to use the Elveflow Smart Interface to set a series of functions and pressure values and add them to the project scheduler:

Be sure that all the cables and tubing are well connected to your Elveflow devices (USB cable, 24V DC, etc).

Perform leakage tests and remove any air bubbles before starting your experiment to ensure a good flow regulation. Knowing what fitting is best suited to your needs is a first step towards success. If you’re not familiar with microfluidic fittings, you may read our specific tutorials.

  • Step 1 – Open the Elveflow® smart interface on the computer by connecting the MUX and the OB1.
  • Step 2 – Select the OB1 (“OB1MixO1” on the example case) and set an initial desired functions and pressure values in mbar for each channel. On the example case we have set:

Channel 1 (Yellow medium): Function sinus, max pressure 160 and min pressure 118 mbar, period 0.7 seconds and dissymmetry 180 (in order to oppose phases) Channel 2 (White medium): Function constant, 120 mbar. Channel 3 (Blue medium): Function sinus, max pressure 160 and min pressure 118 mbar, period 0.7 seconds and dissymmetry 0.

 It is possible to save this configuration for later use by clicking on the “save config” button.
 It is possible to change the channel name by directly editing the channel name display at the left side of the window.
  • Step 3 – In order to add these parameters to the scheduler , click on the “Add step to project” button.
  • Step 4 – On the scheduler window, press “New step” on the scheduler table. Select the action “Wait” and insert the performing time (“wait time“) for the instruments listed before (8 seconds on the example case).
  • Step 5 - Back on the OB1 main window set the next desired functions and pressure values in mbar for each channel. On the example case we have set :

Channel 1 (Yellow medium): Function sinus, max pressure 45 and min pressure 22 mbar, period 0.7 seconds and dissymmetry 0. Channel 2 (White medium): Function constant, 35 mbar. Channel 3 (Blue medium): The same configuration as channel 1 (this time we are not going to oppose phases).

  • Step 6 – Repeat steps 3 and 4.
  • Step 7 – Back on the OB1 main window set the next desired functions and pressure values in mbar for each channel. On the example case we have set :

Channel 1 (Yellow medium): Function square, max pressure 10 and min pressure 6.5 mbar, period 0.5 seconds, dissymmetry 0. Channel 2 (White medium): Function constant, 6.8 mbar. Channel 3 (Blue medium): The same configuration as channel 1, but setting the dissymetry to 180.

  • Step 8 – Repeat steps 3 and 4.
  • Step 9 – Finally, in order to insert a loop to repeat all the steps a desired number of times, press “New step” on the scheduler table and select the action “Go to”. Choose the step 1 to start the loop (“Step to go”:1) and the repeat number (“repeat”:5 in the example case)
  • Step 10 – Press the button “Start” to start performing the project.

If needed, it is possible to see a graph display by clicking on the “open graph display” button. Set the desired maximum and minimum shown parameters on the display window for pressure and flow rate and press the play icon to launch the pressure and flow rate profiles display. Select the channels you want to display by ticking the corresponding boxes on the channel display selection block.

The screenshots corresponding to some of these steps are in the following gallery:

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Piezo electric microfluidics flow control