Droplet_detection_tool_ELVEFLOW_MICROFLUIDICS

DETECTION OF FLUORESCENT DROPLETS

Droplet generation has a wide application range including emulsion production, single cell analysis, drug delivery and nanoparticle synthesis. Droplets can also be used as micro-bioreactors for chemical or biochemical reactions.

During the application, it is essential to track and probe the individual droplets. The Droplet Detector offers a light-based non-invasive, sensitive, high-throughput and convenient way to achieve this goal.

AUTOMATIC DROPLET SIZE REGULATOR - Produce, detect and control the size of your droplets in real-time

AUTOMATIZE MONODISPERSE DROPLET PRODUCTION

The system automatically regulates the droplet size, producing monodisperse droplets, while you take care of the things that matter.

HIGH SPEED OPTICAL DROPLET DETECTOR AND REGULATOR

Droplet detection and size regulation at a production rate of up to 10 kHz

PRODUCE UNIFORM DROPLETS EVERY TIME

Droplets with C.V. < 0.2%

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The Droplet Detector for Microfluidics brings the strength of optical fiber measurement, high optical sensitivity and real-time processing capacity within a compact design. The use of optical fiber for both illumination and light collection largely frees up the space around the experiment and reduces the optical alignment to only one step.

BECOME A BETA-TESTER

We offer to selected customers the opportunity to get our new products at their final stages of development to refine with us their technical characteristics and software functions. In return, you benefit from specific software development best fitted with your needs and reduced prices on our products.

DROPLET CONTROLLER

Attributes

  • High sample rate: 100 000 samples/s
  • Detect ALL droplets: from 10 to 300µm, at 1 Hz to 10 kHz of production rate
  • Compact design: the use of optical fiber allows low footprint and versatile integration
  • Plug & Play: USB computer connection, empowered by the Elveflow software suite (ESI)
  • Interoperability: TTL source trigger

Application fields

Microfluidics, beauty industry, analytical or bio chemistry, biology (single cell encapsulation), real-time inspection (food, pharmaceutics), aerospace, etc.

  • Droplets counting
  • Micro-particles detection
  • Uniform droplet production
  • Microparticle detection
  • Measure fluorescent residence time distribution

Optical detection microfluidic

PROPERTIES

SPECIFICATION

Unity

EXCITATION

Excitation wavelength

365, 470, 530, 590, 625

nm

Fluorescence filter Set

DAPI, FITC, TRITC, Texas Red, Cy3, Cy5, etc

 

Light output
intensity

0-1.5

mW

Pulse duration (pulse mode)

10-107

µs

Modulation (Lock-in mode)

0.05 -10

kHz

ACQUISITION

Acquisition frequency

0-100

kHz

Acquisition resolution

16

bits

Typ. acquisition dynamic

84 dB

dB

 

Reflection

Fluorescence

 

Measurement range

0 – 12

0 – 0.12

µW

Noise equivalent power

10-5

Base

3 x 10-15

W/Hz1/2

 

Hypersensitive (Analog)

0.3 x 10-15

 

 

Hypersensitive (Digital)

10

kcps

Photoelectric sensitivity (532 nm)

0.5 x 106

Base

2 x 109

V/W

 

Hypersensitivity

1010

V/W

Equivalent fluorescence background

(Specified on normal mode, virtually zero in lock-in mode)

 

< 1 nM FITC

 

OPTICS

Fiber core diameter

50, 100, 200, 400

µm

Numerical aperture

0.22 – 0.6

 

Focalized spot diameter

35, 75, 150, 300

µm

MECHANICS

Dimension

35 x 25 x 12

cm3

Detection limit : <1nM FITC (available option with photon counting capability).

Other wavelength and focalized spot diameter available on request.

These specifications are not contractual and can be changed without notice.

 

USB flow sensor software module

Microfluidc flow control software HeartBeat-ESI-2015Thanks to an intuitive interface, the Elveflow® Smart Interface enables the use of Elveflow® instruments from the simplest commands for beginners to the most complex manipulations for experts… read more

 

Control your experiments through C, Python,  Matlab®, Labview® or the Elveflow® Smart Interface. The Elveflow® Smart Interface is a software application offering all the functionalities that microfluidicists need.

Droplet detector-autodropprod-elvesys-startup-innovation-microfluidics

Droplet detection

This video illustrates how the Droplet detector detects and measures the size of the droplets containing fluorescent targets and regulates their size. In the right side, the studied microfluidic chip is visualized showing the generation and transport of droplets. The Droplet detector generates a light spot at the fluidic channel to probe the fluorescence of these droplets as they move across the illuminated area. The collected signal from the droplets is next visualized and analyzed (statistics on the size, speed and fluorescent intensity) on the left panels. Here, such information is fed back to the pressure control system (lower center panel) to adjust to applied pressure and regulate droplet size.

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Optical detection microfluidic application chemical reaction